Biotechnology
(Code No. 045)
DELETED PORTIONS CLASS XII
Under Unit V: Protein and Gene Manipulation
o Chapter 1: Recombinant DNA Technology
Hybridization techniques, DNA library, Site-directed Mutagenesis
o Chapter 2: Protein Structure and Engineering
3-D shape of proteins, Purification of proteins
o Chapter 3: Genomics, Proteomics and Bioinformatics:
Introduction, Genome Sequencing projects, History of bioinformatics, Sequences and nomenclature.
Under Unit VI: Cell Culture and Genetic Manipulation
тАв Chapter 1: Microbial Cell Culture and its Applications:
Scale-up of microbial process, Biosafety issues in microbial technology
тАв Chapter 2: Plant Cell Culture and Applications
Gene transfer Methods in plants
тАв Chapter 3: Animal Cell Culture and Applications
Characterization of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Tissue engineering
Practicals
1. Isolation of genomic DNA (CTAB method)
2. Bacterial transformation using any plasmid
3. Restriction digestion of plasmid DNA &its analysis by gel electrophoresis
Curriculum Deduction Details (Deleted Portion only for the purpose of Annual Examination-2021)This has to be read along with the revised syllabus and also with the Alternative Calendar of NCERT.
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